Beslenme ve Diyetetik Bölümü Tez Koleksiyonuhttps://hdl.handle.net/11655/4682024-03-28T11:15:14Z2024-03-28T11:15:14ZEFFECTS OF NARINGENIN, BERBERINE, AND DELPHINIDIN PHYTOCHEMICAL COMPOUNDS ON BROWNING OF 3T3-L1 PREADIPOCYTE CELLSÖRS DEMET, Elif Didemhttps://hdl.handle.net/11655/347972024-03-20T06:30:57Z2024-03-01T00:00:00ZEFFECTS OF NARINGENIN, BERBERINE, AND DELPHINIDIN PHYTOCHEMICAL COMPOUNDS ON BROWNING OF 3T3-L1 PREADIPOCYTE CELLS
ÖRS DEMET, Elif Didem
Obesity, a complex global health challenge, demands innovative treatment strategies. Plant-derived bioactive compounds, including flavanones, alkaloids, and anthocyanins, hold promise for combating obesity-related complications. This study explored the effects of delphinidin, naringenin, and berberine – compounds with known anti-obesity potential – on 3T3-L1 preadipocyte cells to unravel their impact on browning of adipose cells. This study investigated the impacts of delphinidin, naringenin, and berberine in both free and liposomal forms on the browning process in 3T3-L1 preadipocyte cells. The expression of key browning markers (PRDM16, PPARγ, PGC1-α, CIDEA, and UCP1) was assessed and triglyceride levels were measured to evaluate lipid metabolism. Quantitative Real Time PCR and Elisa analyses were employed to quantify gene and protein expression levels, respectively, with statistical significance determined by p-values (p<0.05). These findings indicate a complex interaction between the tested phytochemicals and adipocyte browning. Free form of delphinidin showed potential in promoting browning through increased PRDM16 expression (p=0.037) but did not significantly affect beige-specific gene expressions or reduce triglyceride levels except during maturation. High-dose liposomal form of naringenin during maturation increased UCP1 gene expression significantly (p=0.035), indicating potential for browning, but did not impact triglyceride levels. Liposomal form of berberine significantly enhanced UCP1 expression (p=0.002) and reduced triglyceride levels in differentiation (p<0.05), suggesting its efficacy in inducing adipocyte browning. Safety assessments, evaluated with cell viability assays (MTT), indicated that all compounds were safe in free form after 24 hours, with adverse effects noted after 48 hours. This study highlights the differential impacts of delphinidin, naringenin, and berberine on the browning of adipocytes, with berberine showing the most consistent and significant effects in promoting browning and affecting lipid metabolism. These findings lay groundwork for anti-obesity therapeutics, yet comprehensive in vitro and in vivo studies are essential to validate and expand the browning potential of delphinidin, naringenin, and berberine to treat obesity.
Key Words: Naringenin, Berberine, Delphinidin, 3T3-L1 Preadipocyte Cells, Brown Adipose Tissue, Obesity
2024-03-01T00:00:00ZCOVID-19 SALGINI SÜRECİNDE ADÖLESANLARDA YEME ZAMANI, YEME DAVRANIŞI VE UYKU KALİTESİNİN OBEZİTE İLE İLİŞKİSİEVREN, Bernahttps://hdl.handle.net/11655/347812024-03-07T12:19:52Z2024-02-15T00:00:00ZCOVID-19 SALGINI SÜRECİNDE ADÖLESANLARDA YEME ZAMANI, YEME DAVRANIŞI VE UYKU KALİTESİNİN OBEZİTE İLE İLİŞKİSİ
EVREN, Berna
Obesity is rapidly increasing in adolescent individuals, and
factors underlying this condition, such as sleep quality, duration and eating behavior
changes, are gaining importance. The aim of the study is to reveal the relationship
between eating time, eating behavior and sleep quality and obesity of adolescent
individuals who were subject to confinement during the Covid-19 epidemic. In the
research conducted with 162 volunteer participants (120 girls, 42 boys) between the
ages of 12-18 living in Izmir, data were collected by survey technique. Questionnaire;
It consists of 8 sections: Personal Information, General Health Status, Anthropometric
Measurements, Nutritional Habits, Physical Activity Status, Three-Factor Eating Test,
Sleep Quality Scale and Sleep Variables Questionnaire. As a result, BMI values before
and after the pandemic were generally in the 5-85 percentile and 85-95 percentile
groups, and it was determined that they didn’t differ according to gender. It was
concluded that the majority of the participants had regular weekday and weekend
meals. It was observed that there was only a very weak positive relationship (r=0.000)
between the BMI of individuals with ≥95. percentile and lunch time. It was found that
there was a very weak negative (r=-0.046) relationship between sleep quality and BMI
before the pandemic, and a very weak positive relationship (r=0.035) after the
pandemic. Among eating behaviors, it was observed that there was only a very weak
positive relationship (r=0.032) between pre-pandemic BMI and the level of conscious
eating restriction. It was concluded that there is a very weak positive relationship
(r=0.016) between body weight change and sleep quality. It was determined that there
was a moderate positive relationship between sleep quality score and uncontrolled
eating level, emotional eating level and hunger sensitivity level for each of them
(r=0.560, r=0.528, r=0.569) and a weak positive relationship between conscious eating
restriction and eating habits (r=0.435). According to the results of the study, it is
recommended to establish quality sleep and nutrition habits and increase physical
activity to prevent obesity in adolescents.
2024-02-15T00:00:00ZTip 2 Diyabet Tanılı Bireylerde Yeme ve Ağırlık Modelleri Anketi-5 (YAMA-5)'in Türkçe Geçerlik ve Güvenirlik Çalışması ve Anketin Diyabet Distress Ölçeği ile İlişkisinin İncelenmesiKeskinkaya, Şevvalhttps://hdl.handle.net/11655/346592024-02-14T08:07:08Z2024-02-08T00:00:00ZTip 2 Diyabet Tanılı Bireylerde Yeme ve Ağırlık Modelleri Anketi-5 (YAMA-5)'in Türkçe Geçerlik ve Güvenirlik Çalışması ve Anketin Diyabet Distress Ölçeği ile İlişkisinin İncelenmesi
Keskinkaya, Şevval
Type 2 Diabetes Mellitus (T2DM), often accompanied by insulin resistance, is a chronic and metabolic disease that causes disorders in carbohydrate, protein and fat metabolism. Especially in individuals with T2DM who have a high body mass index (BMI) and insulin resistance, there is a risk of developing various eating disorders, especially Binge Eating Disorder (BED). The aim of this study was to evaluate the Turkish validity and reliability of The Questionnaire of Eating and Weight Patterns-5 (QEWP-5) as a Binge Eating Disorder (BED) screening tool in individuals with Type 2 diabetes; and to examine the relationship between the questionnaire and some general characteristics of individuals, such as age, gender, BMI and duration of diabetes, and Diabetes Distress Scale (DDS) scores. In order to do this, 200 adults with Type 2 diabetes aged 19-64 participated in the study. The study questionnaire consists of four parts: "General Information", "Eating and Weight Patterns Questionnaire-5 (QEWP-5)", "Eating Disorder Examination Questionnaire (EDE-Q)" and "Diabetes Distress Scale (DDS)" and the questionnaire was administered to the participants via online interview. While the convergent validity of the validated QEWP-5 to Turkish showed a very strong correlation according to the Spearman coefficient (ρ=0,915 and p<0,001); the concurrent validity was found to show a good level of correlation according to the kappa coefficient (κ=0,632 for objective binge eating attack; κ=0,672 for subjective bulimic attack; κ=0,629 for objective bulimic attack). According to the test-retest results, the measurement reliability of QEWP-5 (κ=0,66) was found to be close to the accepted lower limit (κ=0,70). While probable BED was detected according to QEWP-5 in %4 of the individuals participating in the study, no individual was found to have Bulimia Nervosa. No statistically significant relationship was observed between the detection of possible BED in individuals according to QEWP-5 and the individuals' age, gender, educational status, average duration of diabetes, and treatment methods applied for diabetes (p>0,05). On the other hand, both body weight and BMI averages of individuals with possible BED were found to be significantly higher than those without (91,6±13,79/80,7±14,05 kg and 34,7±6,80/30,0±5,38 kg/m2, respectively; p<0,05). Individuals with possible BED according to QEWP-5; the mean of EDE-Q total and subscale scores and the median values of DDS total and subscale scores were found to be significantly higher than those not detected (p<0,05). The results obtained support that QEWP-5 can be used to screen for BED in individuals with Type 2 diabetes.
2024-02-08T00:00:00ZHAFİF ŞİŞMAN/OBEZ PSORİYATİK ARTRİT HASTALARINDA VÜCUT AĞIRLIK YÖNETİMİ ve BİLİŞSEL EGZERSİZ TERAPİ YAKLAŞIMININ İYİLEŞME ÖZELLİKLERİNE ETKİSİNİN ARAŞTIRILMASIÖteleş, Sümeyrahttps://hdl.handle.net/11655/346582024-02-14T08:06:29Z2024-02-02T00:00:00ZHAFİF ŞİŞMAN/OBEZ PSORİYATİK ARTRİT HASTALARINDA VÜCUT AĞIRLIK YÖNETİMİ ve BİLİŞSEL EGZERSİZ TERAPİ YAKLAŞIMININ İYİLEŞME ÖZELLİKLERİNE ETKİSİNİN ARAŞTIRILMASI
Öteleş, Sümeyra
Obesity is one of the most common comorbidities in Psoriatic Arthritis (PsA), an inflammatory arthritis, and is associated with high disease activity, low quality of life, and unfavorable treatment processes. This study aimed to investigate the effect of diet and cognitive exercise therapy approach (BETY) intervention on disease activity and quality of life in overweight/obese PsA patients. The study included 70 obese/slightly obese patients aged 20-65, who were diagnosed with PsA according to CASPAR (Psoriatic Arthritis Classification Criteria) criteria and applied to Hacettepe University Department of Internal Medicine, Department of Rheumatology between 2021-2022. The patients were randomly divided into three groups (diet, n=23; diet+BETY, n=23; control, n=24) and followed for 12 weeks. While patients in the diet group were applied only to a diet program, patients in the diet+BETY group were applied to a diet program together with a biopsychosocial-based exercise (BETY) program. Disease activity assessments, anthropometric measurements, food consumption records, quality of life scale, and BETY-Biopsychosocial Questionnaire (BETY-BQ) scores were recorded and compared at baseline (week 0) and week twelve. Correlation and logistic regression analyses were used to examine the effects of interventions on disease activity scores. The study was completed with 48 patients. A significant decrease in body weight was observed in the diet+BETY (-6.2±1.3 kg, p<0.001) and diet (-6.6±2.6 kg, p<0.001) groups compared to the baseline. After 12 weeks, the Disease Activity for Psoriatic Arthritis (DAPSA) score significantly decreased in both intervention groups (diet+BETY: -10.7±8.1, p<0.001; diet: -7.6±6.7, p<0.001). Regardless of interventions, weight loss (≥5%) increased the chance of achieving Minimal Disease Activity (MDA) (OR: 6.51, 95% CI: 1.09-38.90). These results showed that weight loss and a biopsychosocial-based exercise intervention, alone or in combination, improved PsA symptoms. Multidisciplinary approaches for obese PsA patients may improve the management of the disease and increase the chances of achieving remission.
2024-02-02T00:00:00Z